产品编号:S525
产品描述:Anti-Human Sumo
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Sumo
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: S525
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Sumo recognizes Human, Mouse and Rat Sumo. This polyclonal antibody was purified using affinity chromatography.
Background:
The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist; Sumo antibody will only recognize isoform 1.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Sumo Blocking Peptide (Leinco Prod. No.: S532)
Blocking Peptide
Sumo Blocking Peptide (Leinco Prod. No.: S532)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat Sumo by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HL60 Whole Cell Lysate (Leinco Prod. No.: H1403).
References
1. Dohmen, R. J. (2004) Biochim. Biophys. Acta 1695:113
2. Matunis, M. J. et al. (1996) J. Cell Biol. 135:1457
3. Gill, G. (2005) Curr. Opin. Genet. Dev. 15:536
4. Desterro, J. M. et al. (1998) Mol. Cell 2:233
Related Products
Sumo Blocking Peptide (Leinco Prod. No.: S532)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HL60 Whole Cell Lysate (Leinco Prod. No.: H1403)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
