产品编号:S523
产品描述:Anti-Human SOCS1(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Suppressor of Cytokine Signaling 1 (SOCS1)
Purified No Carrier Protein
(JAK-Binding Protein, JAB)
Polyclonal Antibody
Prod. No.: S523
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Suppressor of Cytokine Signaling 1 (SOCS1) recognizes an epitope near the C-terminus of Human, Mouse and Rat SOCS1. This polyclonal antibody was purified using affinity chromatography.
Background:
The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines.1 SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase.2 SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome.3 It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP.4
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Suppressor of Cytokine Signaling 1 (CT) Blocking Peptide (Leinco Prod. No.: S533)
Blocking Peptide
Suppressor of Cytokine Signaling 1 (CT) Blocking Peptide (Leinco Prod. No.: S533)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat SOCS1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Spleen Tissue Lysate (Leinco Prod. No.: H1410).
References
1. Rakesh, K. et al. (2005) Biochem. Pharm. 70:649
2. O’Shea, J. J. et al. (2002) Cell 109:S121
3. Kile, B. T. et al. (2002) Trends Biochem. Sci. 27:235
4. Mansell, A. et al. (2006) Nat. Immunol. 7:148
Related Products
Suppressor of Cytokine Signaling 1 (CT) Blocking Peptide (Leinco Prod. No.: S533)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Spleen Tissue Lysate (Leinco Prod. No.: H1410)
Anti-Human Suppressor of Cytokine Signaling 1 (NT) (SOCS1) (Leinco Prod. No.: S524)
Suppressor of Cytokine Signaling 1 (NT) Blocking Peptide (Leinco Prod. No.: S534)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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