产品编号:T357
产品描述:Anti-Human TAB1
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human TAB1
Purified No Carrier Protein
(TAK1-binding Protein 1, MAP3K7-interacting Protein 2)
Polyclonal Antibody
Prod. No.: T357
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human TAB1 recognizes human and mouse TAB1. This polyclonal antibody was purified using affinity chromatography.
Background:
TAB1 was identified as a regulator of the MAP kinase kinase kinase TAK1/MAP3K7 (1), which is known to mediate various intracellular signaling pathways, such as those induced by TGF-β and members of the Toll-IL-1R (TIR) superfamily (2), thus acting as an intermediate in both proliferative and innate and adaptive immune responses (3). This protein, together with either TAB2 or TAB3, activates TAK1 kinase in response to upstream signals (4,5). It has been shown that the C-terminal portion of TAB1 is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF-β, demonstrating how this protein can function as a mediator between TGF-β receptors and TAK1 (6).
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
TAB1 Blocking Peptide (Leinco Prod. No.: T362)
Blocking Peptide
TAB1 Blocking Peptide (Leinco Prod. No.: T362)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), WB, In vivo Depletion
Western Blotting: This antibody can be used to detect human and mouse TAB1 by Western blot analysis at a concentration of 0.5 - 1 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is 3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183).
References
1. Shibuya H, Yamaguchi K, Shirakabe K, et al. TAB1: an activator
of the TAK1 MAPKKK in TGF-beta signal transduction. Science
1996; 272:1179-82.
2. Irie T, Muta T, and Takeshige K. TAK1 mediates an activation
signal from toll-like receptor(s) to nuclear factor-kappaB in
lipopolysaccharide-stimulated macrophages. FEBS Lett. 2000;
467:160-4.
3. Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev.
Immunol. 2004; 4:499-511.
4. Jiang Z, Ninomiya-Tsuji J, Qian Y, et al. Interleukin-1 (IL-1)
receptor-associated kinase-dependent IL-1-induced signaling
complexes phosphorylate TAK1 and TAB2 at the plasma
membrane and activate TAK1 in the cytosol. Mol. Cell Biol. 2002;
22:7158-67.
5. Cheung PC, Nebreda AR, and Cohen P. TAB3, a new binding
partner of the protein kinase TAK1. Biochem J. 2004; 378:27-34.
6. Ono K, Ohtomo T, Ninomiya-Tsuji J, et al. A dominant negative
TAK1 inhibits cellular fibrotic responses induced by TGF-beta.
Biochem. Biophys. Res. Commun. 2003; 307:332-7.
(RD0406)
Related Products
TAB1 Blocking Peptide (Leinco Prod. No.: T362)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
