产品编号:T279
产品描述:Anti-Human TIP47(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Tail-Interacting Protein 47 (TIP47)
Purified No Carrier Protein
(Mannose-6-Phosphate Receptor Binding Protein 1, Placental Protein 17)
Polyclonal Antibody
Prod. No.: T279
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Tail-Interacting Protein 47 (TIP47) recognizes an epitope near the C-terminus of Human and Rat TIP47. This polyclonal antibody was purified using affinity chromatography.
Background:
Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.
Known Species Cross Reactivity: Hu+, Rt+
Immunogen
Tail-Interacting Protein 47 (CT) Blocking Peptide (Leinco Prod. No.: T297)
Blocking Peptide
Tail-Interacting Protein 47 (CT) Blocking Peptide (Leinco Prod. No.: T297)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Rat TIP47 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Daudi Whole Cell Lysate (Leinco Prod. No.: D310).
References
1. Barbero, P. et al. (2002) J. Cell Biol. 156:511
2. Carroll, K. S. et al. (2001) Science 292:1373
3. Aivazian, D. et al. (2006) J. Cell Biol. 173:917
4. Ganley, I. G. et al. (2004) Mol. Biol. Cell 15:5420
Related Products
Tail-Interacting Protein 47 (CT) Blocking Peptide (Leinco Prod. No.: T297)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Daudi Whole Cell Lysate (Leinco Prod. No.: D310)
Anti-Human Tail-Interacting Protein 47 (NT) (TIP47) (Leinco Prod. No.: T280)
Tail-Interacting Protein 47 (NT) Blocking Peptide (Leinco Prod. No.: T298)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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