产品编号:T281
产品描述:Anti-Human Target of Rapamycin(TOR)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Target of Rapamycin (TOR)
Purified No Carrier Protein
(mTOR, FRAP, RAFT, RAPT)
Polyclonal Antibody
Prod. No.: T281
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Target of Rapamycin (TOR) recognizes Human and Mouse TOR. This polyclonal antibody was purified using affinity chromatography.
Background:
The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin.2,3 Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 4). TOR is normally associated with the regulatory proteins RAPTOR and GβL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis.4
Known Species Cross Reactivity: Ms+, Hu+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Mouse TOR by Western blot analysis at a concentration of 1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is L1210 Whole Cell Lysate (Leinco Prod. No.: L160).
References
1. Shamji, A. F. et al. (2003) Mol. Cell 12:271
2. Sabatini, D. M. et al. (1994) Cell 78:35
3. Cardenas, M. E. et al. (1995) Curr. Opin. Nephrol. Hypertens. 4:472
4. Fingar, D. C. et al.(2004) Oncogene 23:3151
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
L1210 Whole Cell Lysate (Leinco Prod. No.: L160)
Target of Rapamycin Blocking Peptide (Leinco Prod. No.: T301)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
