产品编号:T360

产品描述:Anti-Human TIRP(CT-2)

反应种属:

实验方法:

标记:

规格:0.5mg

供应商:Leinco

价格(RMB):16095.00

订购:

说明书:

Rabbit Anti-Human TIRP
Purified No Carrier Protein
(TRAM, TICAM-2)
Polyclonal Antibody

Prod. No.: T360
Host:         Rabbit
Conc.:       0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage:     2-8°C

Description
Specificity:
Rabbit Anti-Human TIRP recognizes an epitope near the C-terminus of human, mouse and rat TIRP. This polyclonal antibody was purified using affinity chromatography.

Background:
TIRP is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) (1-3). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses (4). TIRP, along with other molecules such as TRIF, MAL, and MyD88, serves as an adaptor protein that allows for the interaction and activation of the IL-1R-associated kinase (IRAK) family, the subsequent activation of TNF receptor associated factor (TRAF)-6, and ultimately the activation of NF-κB (5). Expression of TIRP appears to be essential for TLR4 signalling (6).

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
TIRP (CT-2) Blocking Peptide (Leinco Prod. No.: T365)
Blocking Peptide
TIRP (CT-2) Blocking Peptide (Leinco Prod. No.: T365)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB, In vivo Depletion
Western Blotting: This antibody can be used to detect human, mouse and rat TIRP by Western blot analysis at a concentration of 0.5 - 2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are Human Kidney Tissue Lysate (Leinco Prod. No.: H1409) Mouse Kidney Tissue Lysate (Leinco Prod. No.: M1017) and Rat Kidney Tissue Lysate (Leinco Prod. No.: R1013).

References
1. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1
receptor adaptor family grows to five members. Trends in
Imm. 2003; 24:286-9.

2. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs:
differential adapter utilization by toll-like receptors mediates
TLR-specific patterns of gene expression. Mol. Interv. 2003;
3:466-77.

3. Takeda K, Kaisho T, and Akira S. Toll-like receptors.
Annu. Rev. Immunol. 2003; 21:335-76.

4. Janeway CA Jr and Medzhitov R. Innate immune
recognition. Annu. Rev. Immunol. 2002; 20:197-216.

5. Bin L-H, Xu L-G, and Shu H-B. TIRP, a novel
toll/interleukin-1 receptor (TIR) domain-containing adaptor
protein involved in TIR signaling. J. Biol. Chem. 2003;
278:24526-24532.

6. Fitzgerald KA, Rowe DC, Barnes, BJ et al. LPS-TLR4
signaling to IRF-3/7 and NF-kB involves the toll adaptors
TRAM and TRIF. J. Exp. Med. 2003; 198:1043-1055.
Related Products
TIRP (CT-2) Blocking Peptide (Leinco Prod. No.: T365)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Kidney Tissue Lysate (Leinco Prod. No.: H1409)
Mouse Kidney Tissue Lysate (Leinco Prod. No.: M1017)
Rat Kidney Tissue Lysate (Leinco Prod. No.: R1013)
Anti-Human TIRP (CT-1) (Leinco Prod. No.: T361)
TIRP (CT-1) Blocking Peptide (Leinco Prod. No.: T366)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.