产品编号:P161
产品描述:Anti-Human p62 DOK
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human Tyrosine Kinase Substrate p62dok (p62 DOK)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: P161
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Tyrosine Kinase Substrate p62dok (p62 DOK) recognizes an epitope near the C-terminus of Human p62 DOK. This polyclonal antibody was purified using affinity chromatography.
Background:
Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.
Known Species Cross Reactivity: Hu+
Immunogen
Tyrosine Kinase Substrate p62dok (CT) Blocking Peptide (Leinco Prod. No.: P188)
Blocking Peptide
Tyrosine Kinase Substrate p62dok (CT) Blocking Peptide (Leinco Prod. No.: P188)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human p62 DOK by Western blot analysis at a concentration of 0.5 to 1 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Jurkat Whole Cell Lysate (Leinco Prod. No.: J103).
References
1. Carpino, N. et al. (1997) Cell 88:197
2. Yamanashi, Y. et al. (1997) Cell 88:205
3. Holland, S. J. et al. (1997) EMBO J. 16:3877
4. Di Cristofano, A. et al. (1998) J. Biol. Chem. 273:4827
Related Products
Tyrosine Kinase Substrate p62dok (CT) Blocking Peptide (Leinco Prod. No.: P188)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Jurkat Whole Cell Lysate (Leinco Prod. No.: J103)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
.jpg)
.jpg)
