产品编号:X101
产品描述:Anti-Human XBP-1(NT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human X Box Binding Protein 1 (XBP-1)
Purified No Carrier Protein
(Tax-Responsive Element Binding Protein 5)
Polyclonal Antibody
Prod. No.: X101
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human X Box Binding Protein 1 (XBP-1) recognizes an epitope near the N-terminus of Human XBP-1. This polyclonal antibody was purified using affinity chromatography.
Background:
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.3,4
Known Species Cross Reactivity: Hu+
Immunogen
X Box Binding Protein 1 (NT) Blocking Peptide (Leinco Prod. No.: X110)
Blocking Peptide
X Box Binding Protein 1 (NT) Blocking Peptide (Leinco Prod. No.: X110)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human XBP-1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is PC-3 Whole Cell Lysate (Leinco Prod. No.: P166).
References
1. Yoshida, H. et al. (2001) Cell 107:881
2. Calfon, M. et al. (2002) Nature 415:92
3. Haze, K. et al. (1999) Mol. Cell. Biol. 10:3787
4. Little, E. et al. (1994) Crit. Rev. Eukaryot. Gene Expr. 4:1
Related Products
X Box Binding Protein 1 (NT) Blocking Peptide (Leinco Prod. No.: X110)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
PC-3 Whole Cell Lysate (Leinco Prod. No.: P166)
Anti-Human X Box Binding Protein 1 (CT) (XBP-1) (Leinco Prod. No.: X100)
X Box Binding Protein 1 (CT) Blocking Peptide (Leinco Prod. No.: X109)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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