产品编号:C1109
产品描述:Anti-Mouse CAD(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Mouse Caspase Activated Deoxyribonuclease (CAD)
Purified No Carrier Protein
(DFF40)
Polyclonal Antibody
Prod. No.: C1109
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Mouse Caspase Activated Deoxyribonuclease (CAD) recognizes an epitope near the C-terminus of Mouse CAD. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease).1,2 The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40.3,4 Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively.1,2,5 Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei.1-4 Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Known Species Cross Reactivity: Ms+
Immunogen
Caspase Activated Deoxyribonuclease (CT) Blocking Peptide (Leinco Prod. No.: C1125)
Blocking Peptide
Caspase Activated Deoxyribonuclease (CT) Blocking Peptide (Leinco Prod. No.: C1125)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Mouse CAD by Western blot analysis at a dilution of 1:500-1:1,000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are PN:M1142 or PN:M1138.
References
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
3. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
4. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40
Related Products
Caspase Activated Deoxyribonuclease (CT) Blocking Peptide (Leinco Prod. No.: C1125)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
PN:M1142
PN:M1138
Caspase Activated Deoxyribonuclease (I17) Blocking Peptide (Leinco Prod. No.: C1126)
DFF40 (I18) Blocking Peptide (Leinco Prod. No.: D270)
Anti-Mouse Caspase Activated Deoxyribonuclease (I17) (CAD) (Leinco Prod. No.: C1145)
PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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