产品编号:I-468
产品描述:Anti-Mouse IRAK2(CT-1)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Mouse IL-1 Receptor Associated Kinase 2 (IRAK2)
Purified No Carrier Protein
(mIRAK2)
Polyclonal Antibody
Prod. No.: I-468
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Mouse IL-1 Receptor Associated Kinase 2 (IRAK2) recognizes an epitope near the C-terminus of Human and Mouse IRAK2. This polyclonal antibody was purified using affinity chromatography.
Background:
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2.1 Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation.2,3 Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, mouse IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion.4 IRAK2 is expressed in a variety of tissues.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
IRAK2(CT-1) (Mouse) Blocking Peptide (Leinco Prod. No.: I-491)
Blocking Peptide
IRAK2(CT-1) (Mouse) Blocking Peptide (Leinco Prod. No.: I-491)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Mouse IRAK2 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is RAW 264.7 Whole Cell Lysate (Leinco Prod. No.: R1174).
References
1. Muzio, M. et al. (1997) Science 278:1612
2. Zhang, F. X. et al. (1999) J. Biol. Chem. 274:7611
3. Yang, R. B. et al. (1999) J. Immunol. 163:639
4. Hardy, M. P. et al. (2004) J. Biol. Chem. 279
Related Products
IRAK2(CT-1) (Mouse) Blocking Peptide (Leinco Prod. No.: I-491)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
RAW 264.7 Whole Cell Lysate (Leinco Prod. No.: R1174)
IRAK2 (CT-1) (Human) Blocking Peptide (Leinco Prod. No.: I-324)
IRAK2 (CT-2) (Human) Blocking Peptide (Leinco Prod. No.: I-323)
Anti-Human IL-1 Receptor Associated Kinase 2 (CT-2) (IRAK-2) (Leinco Prod. No.: I-308)
Anti-Human IL-1 Receptor Associated Kinase 2 (CT-1) (IRAK-2) (Leinco Prod. No.: I-309)
PN:
Anti-Mouse IL-1 Receptor Associated Kinase 2 (CT-2) (IRAK2) (Leinco Prod. No.: I-467)
IRAK2 (CT-2) (Mouse) Blocking Peptide (Leinco Prod. No.: I-492)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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