产品编号:I-501

产品描述:Anti-Human IL-27(NT)

反应种属:

实验方法:

标记:

规格:0.5mg

供应商:Leinco

价格(RMB):17925.00

订购:

说明书:

Rabbit Anti-Human Interleukin-27 (IL-27)
Purified No Carrier Protein
(P28, IL30, IL-27, IL-27p28, IL27)
Polyclonal Antibody

Prod. No.: I-501
Host:          Rabbit
Conc.:     1.0 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:         2-8°C

Description
Specificity:
Rabbit Anti-Human Interleukin-27 (IL-27) recognizes an epitope near the N-terminus of Human and Mouse IL-27. This polyclonal antibody was purified using affinity chromatography.

Background:
Interleukin-27 (IL-27) is a novel IL-12 family member that plays a role in the early regulation of T helper cell 1 initiation, including induction of T-bet and IL-12 receptor beta 2 expression.1 IL-27 is produced by antigen-presenting cells2 and plays an important function in regulating the activity of B and T lymphocytes.3 IL27 may be a potential therapeutic agent against rheumatoid arthritis (RA) at the onset of the disease.4

Known Species Cross Reactivity: Ms+, Hu+
Immunogen
IL-27 (NT) Blocking Peptide (Leinco Prod. No.: I-513)
Blocking Peptide
IL-27 (NT) Blocking Peptide (Leinco Prod. No.: I-513)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human IL-27 this polyclonal antibody can be used at a concentration of 2-4 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is EL4 Whole Cell Lysate (Leinco Prod. No.: E153).

References
1. Yoshimoto T et al. (2004) Cancer Res. 64: 1152
2. De Sauvage FJ et al. (2003) Proceedings of the National Acad of Sci of USA 100: 15047
3. Devergne O et al. (2006) J Immunol. 176: 5890
4. Liew FY et al. (2008) Ann Rheum Dis. 67: 1474
Related Products
IL-27 (NT) Blocking Peptide (Leinco Prod. No.: I-513)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
EL4 Whole Cell Lysate (Leinco Prod. No.: E153)
Anti-Human IL-27 (Intermediate Domain) (Leinco Prod. No.: I-500)
IL-27 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: I-512)
Recombinant Mouse IL-27 (p28) (Leinco Prod. No.: I-811)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.