产品编号:I-508
产品描述:Anti-Human IRF8(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Interferon Regulatory Factor-8 (IRF8)
Purified No Carrier Protein
(Interferon Consensus Sequence Binding Protein, ICSBP)
Polyclonal Antibody
Prod. No.: I-508
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Interferon Regulatory Factor-8 (IRF8) recognizes an epitope near the C-terminus of Human, Mouse and Rat IRF8. This polyclonal antibody was purified using affinity chromatography.
Background:
Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-α/β genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases,2,3 but IRF8 mediates activation of NF-κB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells.4 Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
IRF8 Blocking Peptide (Leinco Prod. No.: I-520)
Blocking Peptide
IRF8 Blocking Peptide (Leinco Prod. No.: I-520)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat IRF8 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is K562 Whole Cell Lysate (Leinco Prod. No.: K128).
References
1. Malmgaard, L. (2004) J. Interferon & Cyto. Res. 24:439
2. Weisz, A. et al. (1992) J. Biol. Chem. 267:25589
3. Nelson, N. et al. (1993) Mol. Cell. Biol. 13:588
4. Tsujimura, H. et al. (2004) J. Immunol. 172:6820
Related Products
IRF8 Blocking Peptide (Leinco Prod. No.: I-520)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
