产品编号:B393
产品描述:Anti-Mouse B7-1
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):4155.00
订购:
说明书:
Goat Anti-Mouse B7-1
Antigen Affinity Purified
(CD80)
Polyclonal Antibody
Prod. No.: B393
Host: Goat
Pkg. Size: 1.0 mg, 50 µg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse B7-1 recognizes Mouse B7-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
It is widely accepted that T cells require a signal induced by the engagement of the T cell receptor and a “costimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counterreceptors were identified by their ability to interact with costimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3 and PD-L2. B7-1 is expressed on activated B cells, activated T cells, mature dendritic cells and macrophages. T cells express two different receptors capable of binding B7-1, CD28 and CTLA-4. CTLA-4 binds to B7-1 with a 20 - 100 fold higher affinity than CD28. Engagement of CD28 by B7-1 increases T cell proliferation and IL-2 production and enhances the immune response. In contrast, engagement of CTLA-4 is involved in the down-regulation of the immune response. Rat and human B7-1 share 47% amino acid identity.
Immunogen
NS0-Derived Recombinant Mouse B7-1 Extracellular Domain
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, FC, N, WB
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing B7-1. A suggested method would be to stain cells expressing B7-1 with 0.50 µg per 1 - 2.5 x 105 cells in a total staining volume of ≤200 µl followed by Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641).
Neutralization: This antibody is useful for neutralization of Mouse B7-1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse B7-1 (at 0.10 ng/ml) is 0.001 – 0.003 µg/ml.
Western Blotting: To detect Mouse B7-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse B7-1 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Related Products
Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641)
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human B7-1 (Antigen Aff. Pur.) (Leinco Prod. No.: B558)
Anti-Human B7-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B602)
Anti-Human B7-1 (Leinco Prod. No.: B566)
Anti-Mouse B7-1 (Leinco Prod. No.: B584)
Anti-Mouse B7-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B394)
Recombinant Mouse B7-1 (Leinco Prod. No.: B544)
Recombinant Human B7-1 (Leinco Prod. No.: B532)
Surfacetag™ Mouse CD Markers
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
