产品编号:I-685
产品描述:Anti-Human IL-2
反应种属:
实验方法:
标记:
规格:500ug
供应商:Leinco
价格(RMB):4875.00
订购:
说明书:
Anti-Human Interleukin-2 (IL-2)
Purified No Carrier Protein
(TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin)
Monoclonal Antibody
Prod. No.: I-685
Clone: 5355
Isotype: Mouse IgG2a
Pkg. Size: 1 mg, 500 µg
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Interleukin-2 (IL-2) (Clone 5355) recognizes an epitope on Human IL-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Interleukin-2 (IL-2) is a potent T-cell mitogenic cytokine1 that functions as a primary regulator of T cell homeostasis and has been considered a prime candidate immunotherapeutics, both for increasing T cell levels/function and for augmenting vaccine-elicited viral-specific T cell responses.2 IL-2 plays a key role in setting the balance between immunity and tolerance. This cytokine has a dual role as the regulator of the two main phases of the immune response (proliferative and suppressive).3 IL-2 also stimulates growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human IL-2 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (NBF/Par.), N
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is Anti-Human IL-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-215) at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is Recombinant Human IL-2 (Leinco Prod. No.: I-184).
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 - 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human IL-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-2 (at 2 ng/ml) is 0.25 - 0.5 µg/ml.
References
1. Schachter J et al. (2009) Cytotherapy 11: 206
2. Sodora DL et al. (2009) Curr HIV Res. 7: 83
3. Alcocer-Varela J et al. (2009) Autoimmun Rev.
Related Products
Anti-Human IL-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-215)
Recombinant Human IL-2 (Leinco Prod. No.: I-184)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
Recombinant Rat IL-2 (Leinco Prod. No.: I-279)
Human IL-2 ELISA Development Kit (Leinco Prod. No.: I-388)
Anti-Human IL-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-427)
Anti-Human IL-2 (Antigen Aff. Pur.) (Leinco Prod. No.: I-426)
Anti-Human IL-2 (Leinco Prod. No.: I-172)
Anti-Mouse IL-2 (Leinco Prod. No.: I-660)
Anti-Mouse IL-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-216)
Anti-Rat IL-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-780)
Anti-Human IL-2 (Antigen Aff. Pur.) (Leinco Prod. No.: I-146)
Anti-Mouse IL-2 (Antigen Aff. Pur.) (Leinco Prod. No.: I-234)
Mouse IL-2 ELISA Development Kit (Leinco Prod. No.: I-284)
Recombinant Mouse IL-2 (Leinco Prod. No.: I-837)
Recombinant Mouse IL-2 (Leinco Prod. No.: I-218)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
