产品编号:T435
产品描述:Anti-Human TRAIL
反应种属:
实验方法:
标记:
规格:500ug
供应商:Leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human TNF-Related Apoptosis-Inducing Ligand (TRAIL)
Purified No Carrier Protein
(TNFSF10, APO2L, Apo-2L, CD253, TL2)
Monoclonal Antibody
Prod. No.: T435
Clone: 75411
Isotype: Mouse IgG1
Pkg. Size: 1mg, 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human TNF-Related Apoptosis-Inducing Ligand (TRAIL) (Clone 75411) recognizes an epitope on Human TRAIL. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
TNF-related apoptosis-inducing ligand (TRAIL) belongs to the tumor necrosis factor family and activates rapid apoptosis in tumor cells, and binds to the death-signaling receptor DR4.1 TRAIL is a potential tumor specific cancer therapeutic agent, since it selectively induces apoptosis in transformed versus normal cells.2
Host Species
Mouse
Immunogen
NS0-Derived Recombinant Human TRAIL Extracellular Domain
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human TRAIL is approximately 25.0 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human TRAIL bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human TRAIL (at 12 ng/ml) is 2-8 µg/ml.
References
1. Ashkenazi A et al. (1997) Science 227: 818
2. Romeo G et al. (2005) Current Pharmaceutical Design 11: 933
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TRAIL (CT) Blocking Peptide (Leinco Prod. No.: T205)
Anti-Human TRAIL (CT) (Leinco Prod. No.: T202)
Anti-Human TRAIL-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T196)
Recombinant Human TRAIL (Leinco Prod. No.: T392)
Anti-Human TRAIL (Antigen Aff. Pur.) (Leinco Prod. No.: T168)
Recombinant Mouse TRAIL (Leinco Prod. No.: T523)
Anti-Mouse TRAIL ( Antigen Aff. Pur. ) (Leinco Prod. No.: T533)
Anti-Mouse TRAIL-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T540)
Anti-Mouse TRAIL (Leinco Prod. No.: T543)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
