产品编号:CYT-561-5ug

产品描述:Human Transforming Growth Factor-Beta 1(TGF-b1)

反应种属:

实验方法:

标记:

规格:5µg

供应商:Prospec

价格(RMB):25200.00

订购:

说明书:

TGF b 1 Human
Transforming Growth Factor-beta 1 Human
Catalog No. CYT-561
 
Synonyms:
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1.

Introduction:
Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.

Description:
Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa.
The TGF-b 1 is purified by proprietary chromatographic techniques.

Source:
Human Platelets

Physical Appearance:
Sterile Filtered lyophilized powder.

Formulation:
Each µg TGF-Beta1 protein contains 10µg Bovine Serum Albumin as a preservative.

Solubility:
It is recommended to reconstitute 5µg lyophilized TGF-beta 1 in sterile 100µl 5mM HCl, which can then be further diluted to the desired aliquot to 1ml of PBS containing 2mg Bovine Serum Albumin.

Stability:
Lyophilized TGF-beta 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGF-beta 1 should be stored at 4°C between 2-7 days and for future use below -18°C.
DO NOT RECONSTITE WITH NEUTRAL BUFFERS.
DO NOT USE GLASS IMPLEMENTS OR EXTENSIVE MANIPULATIONS.
PREVENT FREEZE THAW CYCLES.

Purity:
Greater than 97.0%.

Biological Activity:
Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5ng/ml. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF-alpha at concentrations of at least 2ng/ml must be present for observation of the biological activity.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.