产品编号:CYT-524-2ug
产品描述:Recombinant Human Progranulin(PGRN)
反应种属:
实验方法:
标记:
规格:2µg
供应商:Prospec
价格(RMB):800.00
订购:
说明书:
PGRN Human
Progranulin Human Recombinant
Catalog No. CYT-524
Synonyms:
GRN, PGRN, granulin, Acrogranin, propithelin, PC cell derived growth Factor, GEP, GP88, PEPI, PCDGF.
Introduction:
A 88-kDa progranulin, also called proepithelin and PC cell-derived growth factor, is a single precursor protein of granulins which are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. Granulins are a variety of active, 6 kDa peptides and named granulin A (epithelin 1), granulin B (epithelin 2), granulin C, etc. Both the peptides and intact progranulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Description:
Progranulin Human Recombinant fused to FLAG at C-terminus produced in HEK is a single, glycosylated, polypeptide chain containing 593 amino acids and having a molecular mass of 88 kDa.
The Progranulin is purified by standard chromatographic techniques.
Source:
HEK 293 cells
Physical Appearance:
Sterile Filtered clear solution.
Formulation:
The protein solution contains 1x PBS.
Stability:
Progranulin although stable at 4°C for 3 weeks, should be stored at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Purity:
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity:
1. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in THP-1 cells.
To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degrees Celsius over 0, 30, 60, minutes, respectively by adding the recombinant protein (100ng/ml) to the THP-1 monocyte cell, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 1, 2, and 3 were subjected to THP-1 monocyte cell treatments over 0, 30, 60mins, respectively.
2. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in MCF10A cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degrees Celsius over 0, 10, 30, 60mins, respectively by adding the recombinant protein(500ng/ml) to the MCF10A human breast epithelial cells, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 2, 3, and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 10, 30, 60mins, respectively.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
