产品编号:CYT-445-10ug

产品描述:Recombinant Human Myostatin, His-Tag(Myostatin His)

反应种属:

实验方法:

标记:

规格:10µg

供应商:Prospec

价格(RMB):1820.00

订购:

说明书:

Myostatin Human, His
Myostatin Human Recombinant, His Tag
Catalog No. CYT-445

Synonyms:
GDF-8, MSTN, Growth/Differentiation Factor 8,MSTN Muscle Hypertrophy.

Introduction:
Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor β superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.

Description:
Total 152AA. M.W. 16.7kDa (calculated). N-terminal His-tag and spacer (43AA – highlighted). The AA sequence of the human myostatin part of the fusion protein is corresponding to the UniProtKB/Swiss-Prot entry O14793.
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDPSSRSAVR SRRDFGLDCD EHSTESRCCR YPLTVDFEAFGWDWIIAPKR YKANYCSGEC EFVFLQKYPH THLVHQANPR GSAGPCCTPT KMSPINMLYF NGKEQIIYGKIPAMVVDRCG CS

Source:
Escherichia Coli.

Formulation:
Sterile filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M acetate buffer, pH 4.5.

Solubility:
Add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited.

Purity:
Greater than 95% as determined by SDS-PAGE.

Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized GDF-8 remains stable until the expiry date when stored at -20°C.

Applications:
Western blotting, ELISA.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.