产品编号:CYT-305-1mg
产品描述:Recombinant Human Glial-Derived Neurotrophic Facto
反应种属:
实验方法:
标记:
规格:1mg
供应商:Prospec
价格(RMB):61280.00
订购:
说明书:
GDNF Human
Glial-Derived Neurotrophic Factor Human Recombinant
Catalog No. CYT-305
Synonyms:
ATF1, ATF2, HFB1-GDNF, GDNF.
Introduction:
GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.
GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Description:
Glial derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton.
GDNF is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
GDNF was lyophilized after dialysis against 10mM sodium citrate and 150mM NaCl.
Solubility:
It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.
Biological Activity:
The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml.
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
