产品编号:CYT-440-5ug

产品描述:Mouse beta Nerve Growth Factor(mb-NGF)

反应种属:

实验方法:

标记:

规格:5µg

供应商:Prospec

价格(RMB):800.00

订购:

说明书:

Synonyms:
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.

Introduction:
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

Description:
NGF beta Mouse produced in Submaxillary Gland of Grown Mouse is a homodimer, non-glycosylated, polypeptide chain containing 2 identical 120 amino acids and having a molecular mass of 13,471 Dalton each.
The NGF beta Mouse is purified by advanced biology purification technology.

Source:
Submaxillary Gland of Grown Mouse

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The NGF beta Mouse was lyophilized from solution containing 5% mannitol and 1% HSA.

Solubility:
It is recommended to reconstitute the lyophilized Murine NGF-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Mouse Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Murine NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 98% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE .

Amino acid sequence:
SSTHPVFHMGEF SVCDSVSVWV GDKTTATDIK GKEVTVLAEV NINNSVFRQY FFETKCRASN PVESGCRGID SKHWNSYCTT THTFVKALTT DEKQAAWRFI RIDTACVCVL SRKATRRG

Biological Activity:
The method used to test the bioassay is the NGF-dependent survival of dorsal root ganglia neurons of chick embryo, corresponding to a Specific Activity of 5 x 10⁵ IU/mg.

Reference:
V aron S, Raibo rn C. Dissociation, fractionation and culture ofchick embryo sympathetic ganglionic cells [ J ]. J N eu rocy tol, 1972; 1: 211- 221

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.