产品编号:W148

产品描述:Anti-West Nile Virus Matrix

反应种属:Virus

实验方法:ELISA

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Virus West Nile Virus Matrix (WNV Matrix)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: W148
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1mg, 0.1mg, 0.5mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-West Nile Virus Matrix (WNV Matrix) recognizes an epitope in the intermediate domain of WNV Matrix. This polyclonal antibody was purified using affinity chromatography.

Background:
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state (reviewed in 1). Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins.1 However, when the viruses are inside of infected cells, the matrix protein exists in its “pre-M” form as a heterodimer with the envelope proteins. Cleavage of the “pre-M” protein to its mature form occurs during release of the virus; this cleavage leas to the dissociation of the heterodimers.2 The WNV receptor has recently been identified as alpha v beta 3 integrin.3

Known Species Cross Reactivity: Virus
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
Indirect ELISA
Indirect ELISA: This antibody can be used at 1.0 µl/ml with a suitable secondary reagent such as Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115). The detection sensitivity for WNV Matrix is approximately 10 ng/well.

References
1. Gould, L. H. et al. (2004) J. Clin. Invest. 113:1102
2. Wengler, G. et al. (1989) J. Virol. 2521
3. Chu, J. J. et al. (2004) J. Biol. Chem. 279:54533
Related Products
Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115)
Anti-West Nile Virus Matrix (CT) (WNV Matrix) (Leinco Prod. No.: W147)

Products are for research use only. Not for use in diagnostic or therapeutic procedures