产品编号:W144
产品描述:Anti-West Nile Virus Envelope Protein(CT)(WNV Env)
反应种属:Virus
实验方法:ELISA
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Virus West Nile Virus Envelope Protein (WNV Env)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: W144
Host: Rabbit
Conc.: 0.2mg/ml
Pkg. Size: 1mg, 0.1mg, 0.5mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-West Nile Virus Envelope Protein (WNV Env) recognizes an epitope near the C-terminus of WNV Env. This polyclonal antibody was purified using affinity chromatography.
Background:
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state (reviewed in 1). Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins.1 While the viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway,2 the highly glycosylated envelope protein plays a major role for WNV entry into target cells as this entry can be inhibited by using a recombinant domain III from the envelope glycoprotein.3 The WNV receptor has recently been identified as alpha v beta 3 integrin.4
Known Species Cross Reactivity: Virus
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
Indirect ELISA
Indirect ELISA: This antibody can be used at 1.0 µl/ml with a suitable secondary reagent such as Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115). The detection sensitivity for WNV Env is approximately 10 ng.
References
1. Gould, L. H. et al. (2004) J. Clin. Invest. 113:1102
2. Yang, J. S. et al. (2002) Emerg. Infect. Dis. 8:1379
3. Chu, J. J. et al. (2005) J. Gen. Virol. 86:405
4. Chu, J. J. et al. (2004) J. Biol. Chem. 279:54533
Related Products
Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115)
Anti-West Nile Virus Envelope Protein (NT) (WNV Env) (Leinco Prod. No.: W145)
Anti-West Nile Virus Envelope Protein (Intermediate Domain) (WNV Env) (Leinco Prod. No.: W146)
West Nile Virus Envelope Protein (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: W151)
West Nile Virus Envelope Protein (NT) Blocking Peptide (Leinco Prod. No.: W152)
West Nile Virus Envelope Protein (CT) Blocking Peptide (Leinco Prod. No.: W153)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
