产品编号:W143

产品描述:Anti-West Nile Virus Core(NT)(WNV Core)

反应种属:Virus

实验方法:ELISA

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Virus West Nile Virus Core (WNV Core)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: W143
Host:      Rabbit
Conc.:     0.5mg/ml
Pkg. Size: 1mg, 0.1mg, 0.5mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-West Nile Virus Core (WNV Core) recognizes an epitope near the N-terminus of WNV Core. This polyclonal antibody was purified using affinity chromatography.

Background:
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state (reviewed in 1). Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins1 which play a major role for WNV entry into target cells.2,3 The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.4

Known Species Cross Reactivity: Virus
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
Indirect ELISA
Indirect ELISA: This antibody can be used at 1.0 µl/ml with a suitable secondary reagent such as Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115). The detection sensitivity for WNV Core is approximately 10 ng.

References
1. Gould, L. H. et al. (2004) J. Clin. Invest. 113:1102
2. Chu, J. J. et al. (2005) J. Gen. Virol. 86:405
3. Chu, J. J. et al. (2004) J. Biol. Chem. 279:54533
4. Yang, J. S. et al. (2002) Emerg. Infect. Dis. 8:1379
Related Products
Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115)
Anti-West Nile Virus Core (CT) (WNV Core) (Leinco Prod. No.: W142)

Products are for research use only. Not for use in diagnostic or therapeutic procedures