产品编号:T334
产品描述:Anti-Human TRIM5γ
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human TRIM5γ
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: T334
Host: Rabbit
Conc.: 0.5mg/ml
Pkg. Size: 1 mg, 0.1mg, 0.5mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human TRIM5γ recognizes Human TRIM5γ. This polyclonal antibody was purified using affinity chromatography.
Background:
TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region.1 TRIM5 has five alternately spliced isoforms, the longest of which is the variant which also contains a carboxy-terminal B30.2 (SPRY) domain.1 While a function has not yet been assigned to TRIM5γ, it is known that expression of TRIM5γ variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1,2,3 albeit at differing efficiencies. Furthermore, the TRIM5γ isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.4
Known Species Cross Reactivity: Hu+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human TRIM5g by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Bladder Tissue Lysate (Leinco Prod. No.: B432).
References
1. Reymond, A. et al. (2001) EMBO J. 20:2140
2. Stremlau, M. et al. (2004) Nature 427:848
3. Hatziioannou, T. et al. (2004) Proc. Nat’l. Acad. Sci. USA 101:10774
4. Xu, L. et al. (2003) Exp. Cell Res. 288:84
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Bladder Tissue Lysate (Leinco Prod. No.: B432)
TRIM5g Blocking Peptide (Leinco Prod. No.: T340)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
