产品编号:T345
产品描述:Anti-Human TIM-1(NT)
反应种属:mouse, human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human TIM-1
Purified No Carrier Protein
(T Cell Immunoglobulin and Mucin Domain Containing Protein 1, Hepatitis A Virus Cellular Receptor 1, HAVcr-1, Kim-1)
Polyclonal Antibody
Prod. No.: T345
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human TIM-1 recognizes an epitope near the N-terminus of Human and Mouse TIM-1. This polyclonal antibody was purified using affinity chromatography.
Background:
The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
TIM-1 (NT) Blocking Peptide (Leinco Prod. No.: T349)
Blocking Peptide
TIM-1 (NT) Blocking Peptide (Leinco Prod. No.: T349)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human TIM-1 this polyclonal antibody can be used at a concentration of 1-2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Feigelstock, D. et al. (1998) J. Virol. 72:6621
2. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135
3. Meyers, J. H. et al. (2005) Trends Mol. Med. 11:362
4. Meyers, J. H. et al. (2005) Nat. Immunol. 6:455
Related Products
TIM-1 (NT) Blocking Peptide (Leinco Prod. No.: T349)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human TIM-1 (Intermediate Domain) (Leinco Prod. No.: T344)
TIM-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: T348)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
