产品编号:T344

产品描述:Anti-Human TIM-1

反应种属:mouse, human, rat

实验方法:WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Rabbit Anti-Human TIM-1
Purified No Carrier Protein
(T Cell Immunoglobulin and Mucin Domain Containing Protein 1, Hepatitis A Virus Cellular Receptor 1, HAVcr-1, Kim-1)
Polyclonal Antibody
Prod. No.: T344
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Human TIM-1 recognizes an epitope in the intermediate domain of Human, Mouse and Rat TIM-1. This polyclonal antibody was purified using affinity chromatography.

Background:
The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
TIM-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: T348)
Blocking Peptide
TIM-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: T348)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat TIM-1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Uterus Tissue Lysate (Leinco Prod. No.: U124).

References
1. Feigelstock, D. et al. (1998) J. Virol. 72:6621
2. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135
3. Meyers, J. H. et al. (2005) Trends Mol. Med. 11:362
4. Meyers, J. H. et al. (2005) Nat. Immunol. 6:455
Related Products
TIM-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: T348)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Uterus Tissue Lysate (Leinco Prod. No.: U124)
TIM-1 (NT) Blocking Peptide (Leinco Prod. No.: T349)
Anti-Human TIM-1 (NT) (Leinco Prod. No.: T345)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.