产品编号:R1244
产品描述:Anti-Human RIG-1
反应种属:mouse, human, rat
实验方法:WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human RIG-1
Purified No Carrier Protein
(Retinoic Acid Inducible Gene 1 Protein, RIG-I, Dead Box Protein 58, ddx58)
Polyclonal Antibody
Prod. No.: R1244
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human RIG-1 recognizes Human RIG-1. This polyclonal antibody was purified using affinity chromatography.
Background:
The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses (1). Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection (2,3). Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKε through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons (4). RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus (5).
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB, In vivo Depletion
Western Blotting: This antibody can be used to detect Human RIG-1 by Western blot analysis at a concentration of 0.5 - 1 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is C2C12 Whole Cell Lysate (Leinco Prod. No.: C1181).
References
1. Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.
2. Yoneyama M, Kikuchi M, Natsukawa T, et al. The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol. 2004; 5:730-7.
3. Alexopoulou L, Holt AC, Medzhitov R, et al. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature 2001; 413:732-8.
4. Sharma S, tenOever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.
5. Kato H, Takeuchi O, Sato S, et al. Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature 2006; 441:101-5. (06-01D)
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Antigen Affinity Purified Polyclonal Antibodies To Growth Factors & Cytokines
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
