产品编号:D325

产品描述:Anti-Human DDX3(Intermediate Domain)

反应种属:mouse, human, rat

实验方法:IHC, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Rabbit Anti-Human DDX3
Purified No Carrier Protein
(Dead Box Polypeptide 3 X-Linked, DBX, Helicase-Like Protein 2 )
Polyclonal Antibody
Prod. No.: D325
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Human DDX3 recognizes an epitope in the intermediate domain of Human, Mouse and Rat DDX3. This polyclonal antibody was purified using affinity chromatography.

Background:
DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases.1,2 DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome.3 DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation.4 It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat DDX3 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HepG2 Whole Cell Lysate (Leinco Prod. No.: H1404).

References
1. Linder, P. et al. (1989) Nature 337:121
2. Park, S. H. et al. (1998) Cytogenet. Cell Genet. 81:178
3. Lahn, B. T. et al. (1997) Science 278:675
4. Abdelhaleem, M. (2005) Clin. Biochem. 38:499
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HepG2 Whole Cell Lysate (Leinco Prod. No.: H1404)
Anti-Human DDX3 (NT) (Leinco Prod. No.: D326)
DDX3 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: D327)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.