产品编号:D288

产品描述:Anti-Human DC-SIGN

反应种属:human

实验方法:IHC, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Human DC-SIGN
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: D288
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Human DC-SIGN recognizes an epitope in the extracellular domain of Human DC-SIGN. This polyclonal antibody was purified using affinity chromatography.

Background:
Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes,1 where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN.2 ICAM-3 is the natural ligand for DC-SIGN.3 A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently.4 DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.

Known Species Cross Reactivity: Hu+
Immunogen
DC-SIGN (Extracellular Domain) Blocking Peptide (Leinco Prod. No.: D289)
Blocking Peptide
DC-SIGN (Extracellular Domain) Blocking Peptide (Leinco Prod. No.: D289)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human DC-SIGN by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Placenta Tissue Lysate (Leinco Prod. No.: P165).

References
1. Geijtenbeek, T. B. et al. (2000) Cell 100:587
2. Pohlmann, S. et al. (2001) J. Virol. 75(10):4664
3. Geijtenbeek, T. B. et al. (2000) Cell 100(5):575
4. Soilleux, E. J. et al. (2000) J. Immunol. 165(6):2937
Related Products
DC-SIGN (Extracellular Domain) Blocking Peptide (Leinco Prod. No.: D289)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Placenta Tissue Lysate (Leinco Prod. No.: P165)
DC-SIGN (CT) Blocking Peptide (Leinco Prod. No.: D287)
Anti-Human DC-SIGN (CT) (Leinco Prod. No.: D286)
Anti-Human DC-SIGN-Fluorescein-(FITC) (Leinco Prod. No.: D367)
Anti-Human DC-SIGN R-phycoerythrin-(R-PE) (Leinco Prod. No.: D368)

Products are for research use only. Not for use in diagnostic or therapeutic procedures