产品编号:A293
产品描述:Anti-Human APOBEC3G
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):6195.00
订购:
说明书:
Rabbit Anti-Human APOBEC3G
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: A293
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human APOBEC3G recognizes an epitope near the N-terminus of Human APOBEC3G. This polyclonal antibody was purified using affinity chromatography.
Background:
The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates.1 Similar to TRIM5α which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection2,3 as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome.4 This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.
Known Species Cross Reactivity: Hu+
Immunogen
APOBEC3G Blocking Peptide (Leinco Prod. No.: A320)
Blocking Peptide
APOBEC3G Blocking Peptide (Leinco Prod. No.: A320)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human APOBEC3G by Western blot analysis at a concentration of 5.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Caco-2 Whole Cell Lysate (Leinco Prod. No.: C1182).
References
1. Jarmuz, A. et al. (2002) Genomics 79:285
2. Stremlau, M. et al. (2004) Nature 427:848
3. Bieniasz, P. D. (2004) Nat. Immunol. 5:1109
4. Sheehy, A. M. et al. (2002) Nature 418:646
Related Products
APOBEC3G Blocking Peptide (Leinco Prod. No.: A320)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Caco-2 Whole Cell Lysate (Leinco Prod. No.: C1182)
Polyclonal Antibodies for Apoptosis & Cell Biology
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
