产品编号:A283

产品描述:Anti-Anthrax Lethal Factor(CT)

反应种属:bacteria

实验方法:ELISA

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Anthrax Anthrax Lethal Factor (Anthrax LF)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: A283
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Anthrax Lethal Factor (Anthrax LF) recognizes an epitope near the C-terminus of Anthrax LF. This polyclonal antibody was purified using affinity chromatography.

Background:
Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF) (1,2 for review). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR)3 and capillary morphogenesis protein 2 (CMG2).4 although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. LF is the primary toxin of anthrax and functions as a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near their amino terminus, interfering with MAPK signaling and inducing apoptosis.

Known Species Cross Reactivity: Bacteria
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
Indirect ELISA

References
1. Schwartz, M. N. (2001) New Engl. J. Med. 345:1621
2. Moayeri, M. et al. (2004) Curr. Opin. Microbiol. 7:19
3. Bradley, K. A. et al. (2001) Nature 414:225
4. Scobie, H. M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170
Related Products
Anti-Anthrax Lethal Factor (Intermediate Domain) (Leinco Prod. No.: A284)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.