产品编号:A282

产品描述:Anti-Anthrax Edema Factor(Anthrax EF)

反应种属:bacteria

实验方法:ELISA

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Anthrax Anthrax Edema Factor (Anthrax EF)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: A282
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Anthrax Edema Factor (Anthrax EF) recognizes Anthrax EF. This polyclonal antibody was purified using affinity chromatography.

Background:
Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF) (1,2 for review). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR)3 and capillary morphogenesis protein 2 (CMG2),4 although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. EF is a calmodulin and Ca++-dependent adenylate cyclase responsible for the edema seen in the disease. It is thought to benefit the B. anthracis bacteria by inhibiting cells of the host immune system.

Known Species Cross Reactivity: Bacteria
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
Indirect ELISA

References
1. Schwartz, M. N. (2001) New Engl. J. Med. 345:1621
2. Moayeri, M. et al. (2004) Curr. Opin. Microbiol. 7:19
3. Bradley, K. A. et al. (2001) Nature 414:225
4. Scobie, H. M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170
Products are for research use only. Not for use in diagnostic or therapeutic procedures.