产品编号:P205

产品描述:Anti-Human PEN2(CT)

反应种属:human

实验方法:WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Rabbit Anti-Human PEN2
Purified No Carrier Protein
(Presenilin Enhancer Protein 2, Gamma-Secretase Subunit PEN-2)
Polyclonal Antibody
Prod. No.: P205
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Human PEN2 recognizes an epitope near the C-terminus of human, mouse and rat PEN2. This polyclonal antibody was purified using affinity chromatography.

Background:
PEN2, in addition to presenilin, nicastrin, and APH-1 forms the γ-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer’s disease(1,2 for review). Like the tumor necrosis factor-α-converting enzyme (TACE) and the β-site cleavage enzyme (BACE) protease families, γ-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the α and γ cleavage site) or the toxic A/β amyloid peptide (from the β and γ cleavage site)(3). It is thought that accumulation of the Aβ peptide is the precursor to Alzheimer’s disease(4).

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
PEN2 (CT) Blocking Peptide (Leinco Prod. No.: P215)
Blocking Peptide
PEN2 (CT) Blocking Peptide (Leinco Prod. No.: P215)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect human, mouse and rat PEN2 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is K562 Whole Cell Lysate (Leinco Prod. No.: K128).

References
1. Weihofen, A. et al. (2003) Trends Cell Biol. 13:71
2. Periz, G. et al. (2004) J. Neurosci. Res. 77:309
3. Selkoe, D. J. (1998) Trends Cell Biol. 8:447
4. Selkoe, S. J. (1999) Nature 399:A23
Related Products
PEN2 (CT) Blocking Peptide (Leinco Prod. No.: P215)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
Anti-Human PEN2 (NT) (Leinco Prod. No.: P206)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.