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Anti-Human Nicastrin(CT)

产品编号:N153

产品描述:Anti-Human Nicastrin(CT)

反应种属:mouse, human, rat

实验方法:WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5700.00

订购:订购

说明书:

Rabbit Anti-Human Nicastrin
Purified No Carrier Protein
(Anterior Pharynx Defective 2, APH-2)
Polyclonal Antibody
Prod. No.: N153
Host:      Rabbit
Conc.:     1.0 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Human Nicastrin recognizes an epitope near the C-terminus of Human, Mouse and Rat Nicastrin. This polyclonal antibody was purified using affinity chromatography.

Background:
Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the γ-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer’s disease(1,2 for review). Like the tumor necrosis factor-a-converting enzyme (TACE) and the b-site cleavage enzyme (BACE) protease families, γ-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the α and γ cleavage site) or the toxic Aβ amyloid peptide (from the β and γ cleavage site)(3). It is thought that accumulation of the Aβ peptide is the precursor to Alzheimer’s disease(4). Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality(5).

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Nicastrin (CT) Blocking Peptide (Leinco Prod. No.: N164)
Blocking Peptide
Nicastrin (CT) Blocking Peptide (Leinco Prod. No.: N164)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human Nicastrin this polyclonal antibody can be used at a concentration of 1 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

References
1. Weihofen A and Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 2003; 13:71-8.

2. Periz G and Fortini ME. Functional reconstitution of γ-secretase through coordinated expression of presenilin, Nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.

3. Selkoe DJ. The cell biology of β-amyloid precursor protein and presenilin in Alzheimer’s disease. Trends Cell Biol. 1998; 8:447-53.

4. Selkoe SJ. Translating cell biology into therapeutic advances in Alzheimer’s disease. Nature 1999; 399:A23-31.

5. Nguyen V, Hawkins C, Bergeron C, et al. Loss of nicastrin elicits an apoptotic phenotype in mouse embryos. Brain Res. 2006; 1086:76-84.
Related Products
Nicastrin (CT) Blocking Peptide (Leinco Prod. No.: N164)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human Nicastrin (Intermediate Domain) (Leinco Prod. No.: N154)
Nicastrin (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: N165)

Products are for research use only. Not for use in diagnostic or therapeutic procedures