产品编号:K129

产品描述:Anti-Human KLHL1

反应种属:human, rat

实验方法:IHC, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Rabbit Anti-Human Kelch-Like 1 Protein (KLHL1)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: K129
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Human Kelch-Like 1 Protein (KLHL1) recognizes an epitope near the N'terminus of human and rat KLHL1. This polyclonal antibody was purified using affinity chromatography.

Background:
The mammalian Kelch-like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues.1,2 The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin-organizing proteins.3 Based on the presence of anti-sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.1

Known Species Cross Reactivity: Hu+, Rt+
Immunogen
Kelch-like 1 Protein Blocking Peptide (Leinco Prod. No.: K130)
Blocking Peptide
Kelch-like 1 Protein Blocking Peptide (Leinco Prod. No.: K130)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human and rat KLHL1 by Western blot analysis at a concentration of 2 -4 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Brain Tissue Lysate (Leinco Prod. No.: H1407).

References
1. Nemes, J. P. et al. (2000) Human Mol. Gen. 9:1543
2. Adams, J. et al. (2000) Trends Cell Biol. 10:17
3. Robinson, D. N. et al. (1997) J. Cell Biol. 138:799
Related Products
Kelch-like 1 Protein Blocking Peptide (Leinco Prod. No.: K130)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Brain Tissue Lysate (Leinco Prod. No.: H1407)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.