产品编号:C1298
产品描述:Anti-Human Clusterin
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Clusterin
Purified No Carrier Protein
(Complement Lysis Inhibitor, CLI, Apolipoprotein J, ApoJ)
Polyclonal Antibody
Prod. No.: C1298
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Clusterin recognizes Human Clusterin. This polyclonal antibody was purified using affinity chromatography.
Background:
Clusterin, also known as Apolipoprotein J (ApoJ), is a ubiquitous multifunctional glycoprotein that can interact with a broad spectrum of molecules such as complement components, various receptors, and the Alzheimer’s b-amyloid peptide.1 Clusterin expression is increased in Alzheimer’s disease brain tissue and clusterin-immunoreactive amyloid plaques are found associated with phospho-tau-positive dystrophic neurites2 and it has been suggested that clusterin facilitates the conversion of diffuse b-amyloid deposits into amyloid and enhances tau phosphorylation in neurites around these plaques. Other reports show that clusterin expression is decreased in proliferating cells and is upregulated in quiescent and senescent cells, suggesting that it may also play a role in aging and tumorigenesis suppression.3 Clusterin exists in at least two distinct isoforms.
Known Species Cross Reactivity: Hu+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), IHC (Frozen), WB
Western Blotting: This antibody can be used to detect Human Clusterin by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Brain Tissue Lysate (Leinco Prod. No.: H1407).
References
1. Calero, M. et al. (2005) Biochem. 38:273
2. Martin-Rehrmann, M. D. et al. (2005) Neurotox. Res. 7:231
3. Trougakos, I. P. et al. (2002) Int. J. Biochem. Cell Biol. 34:1430
4. Martinon, F. et al. (2005) TRENDS Imm. 26:447
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Brain Tissue Lysate (Leinco Prod. No.: H1407)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
