产品编号:B362

产品描述:Anti-Human BACE

反应种属:mouse, human

实验方法:IHC-F, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Human Beta Site APP Cleaving Enzyme (BACE)
Purified No Carrier Protein
(Asp2)
Polyclonal Antibody
Prod. No.: B362
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Human Beta Site APP Cleaving Enzyme (BACE) recognizes recognizes an epitope near the C-terminus of Human and Mouse BACE. This polyclonal antibody was purified using affinity chromatography.

Background:
Accumulation of the amyloid-b (Ab) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. Ab peptide is generated by proteolytic cleavage of the b-amyloid protein precursor (APP) at b- and g-sites by two proteases. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all suffers of Alzheimer’s disease. The long-sought b-secretase was recently identified by several groups independently and designated β-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2).1-4 BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Beta Site APP Cleaving Enzyme (CT) Blocking Peptide (Leinco Prod. No.: B365)
Blocking Peptide
Beta Site APP Cleaving Enzyme (CT) Blocking Peptide (Leinco Prod. No.: B365)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), WB, In vivo Depletion
Western Blotting: This antibody can be used to detect Human and Mouse BACE by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Mouse Brain Tissue Lysate (Leinco Prod. No.: M1015).

References
1. Vassar, R. et al. (1999) Science 286:735
2. Hussain, I. et al. (1999) Mol. Cell Neurosci. 14:419
3. Yan, R. et al. (1999) Nature 402:533
4. Sinha, S. et al. (1999) Nature 402:537

Related Products
Beta Site APP Cleaving Enzyme (CT) Blocking Peptide (Leinco Prod. No.: B365)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Mouse Brain Tissue Lysate (Leinco Prod. No.: M1015)
PN:

Products are for research use only. Not for use in diagnostic or therapeutic procedures.