产品编号:A163

产品描述:Anti-Human β-Amyloid Precursor Protein(CT)(APP)

反应种属:mouse, human, rat

实验方法:IHC, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:

说明书:

Rabbit Anti-Human β-Amyloid Precursor Protein (APP)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: A163
Host:      Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:   2-8°C

Description
Specificity:
Rabbit Anti-Human β-Amyloid Precursor Protein (APP) recognizes an epitope near the C-terminus of Human, Mouse and Rat β-Amyloid Precursor Protein. This polyclonal antibody was purified using affinity chromatography.

Background:
Accumulation of the amyloid-β peptide (αβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The b-amyloid protein precursor (APP) is cleaved by β-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-β peptide (αβ), which is deposited in the brains of all suffers of Alzheimer’s disease.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
β-Amyloid Precursor Protein (CT) Blocking Peptide (Leinco Prod. No.: A281)
Blocking Peptide
β-Amyloid Precursor Protein (CT) Blocking Peptide (Leinco Prod. No.: A281)
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat APP by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Mouse Brain Tissue Lysate (Leinco Prod. No.: M1015).

References
1. Ponte, P. et al. (1988) Nature 331:525
2. Selkoe, D. J. (1994) Annu. Rev. Cell Biol. 10:373
Related Products
β-Amyloid Precursor Protein (CT) Blocking Peptide (Leinco Prod. No.: A281)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Mouse Brain Tissue Lysate (Leinco Prod. No.: M1015)
Anti-Human β-Amyloid Precursor Protein (AβNT) (APP) (Leinco Prod. No.: A162)
β-Amyloid Precursor Protein (AβNT) Blocking Peptide (Leinco Prod. No.: A280)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.