产品编号:I-253
产品描述:Anti-Rat IL-4-Biotin
反应种属:rat
实验方法:WB, ELISA., ICC
标记:
规格:50ug
供应商:leinco
价格(RMB):5985.00
订购:
说明书:
Goat Anti-Rat Interleukin-4 (IL-4)
Biotin
(BCGF, BCDF, B Cell Stimulating Factor, BSF-1)
Polyclonal Antibody
Prod. No.: I-253
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Rat Interleukin-4 (IL-4) recognizes Rat IL-4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption.1 IL-4 is critical for inducing allergic responses.2 It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.3
Known Species Cross Reactivity: Rt+
Immunogen
E. coli-Derived Recombinant Rat Interleukin-4
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB, ICC
Western Blotting: To detect Rat IL-4 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Rat IL-4 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Immunocytochemistry: This antibody will detect IL-4 in cells. The working dilution is 15 μg/mL.
References
1. J Wesley Pike et al. (2001) PNAS 98: 2443
2. Fred D. Finkelman et al. (2003) J Immunol. 170: 3835
3. Kaplan MH et al. (2008) J Immunol. 180: 3551
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IL-4 (Leinco Prod. No.: I-186)
Anti-Mouse IL-4 (Antigen Aff. Pur.) (Leinco Prod. No.: I-164)
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Anti-Mouse IL-4-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-252)
Anti-Human IL-4-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-251)
Anti-Rat IL-4 (Antigen Aff. Pur.) (Leinco Prod. No.: I-225)
Recombinant Mouse IL-4 (Leinco Prod. No.: I-207)
Anti-Human IL-4 (Leinco Prod. No.: I-173)
Anti-Human IL-4 (Leinco Prod. No.: I-631)
Anti-Mouse IL-4 (Leinco Prod. No.: I-662)
Anti-Rat IL-4 (Leinco Prod. No.: I-673)
Anti-Porcine IL-4 (Leinco Prod. No.: I-689)
Anti-Canine IL-4 (Leinco Prod. No.: I-703)
Human IL-4 ELISA Development Kit (Leinco Prod. No.: I-287)
Mouse IL-4 ELISA Development Kit (Leinco Prod. No.: I-288)
Recombinant Rat IL-4 (Leinco Prod. No.: I-221)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
