产品编号:I-248

产品描述:Anti-Rat IL-1β-Biotin

反应种属:rat

实验方法:WB, ELISA., ICC

标记:

规格:50ug

供应商:leinco

价格(RMB):5985.00

订购:

说明书:

Goat Anti-Rat Interleukin-1β (IL-1β)
Biotin
(Catabolin, Lymphocyte-activating factor [LAF], Endogenous Pyrogen [EP], Leukocyte Endogenous Mediator [LEM], Mononuclear Cell Factor [MCF])
Polyclonal Antibody
Prod. No.: I-248
Host:      Goat
Pkg. Size: 1 mg, 50ug
Storage:   -20°C to -70°C

Description
Specificity:
Goat Anti-Rat Interleukin-1β (IL-1β) recognizes Rat IL-1β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
IL-1β is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.

Known Species Cross Reactivity: Rt+
Immunogen
Purified Recombinant Rat IL-1β (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB

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Products are for research use only. Not for use in diagnostic or therapeutic procedures