产品编号:G144
产品描述:Anti-Rat CXCL1-Biotin
反应种属:rat
实验方法:WB
标记:
规格:50ug
供应商:leinco
价格(RMB):5985.00
订购:
说明书:
Goat Anti-Rat CXCL1
Biotin
(GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1)
Polyclonal Antibody
Prod. No.: G144
Host: Goat
Pkg. Size: 1mg, 50ug
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Rat CXCL1 recognizes Rat CXCL1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3,4
Known Species Cross Reactivity: Rt+
Immunogen
E. coli-Derived Recombinant Rat CINC-1
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Rat GRO/KC this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Rat GRO/KC is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Sager R et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732
2. Grotendorst GR et al. (1990) Mol. Cell. Biol. 10: 5596
3. Miller RH et al. (2002) Cell 110: 373
4. Richmond A et al. (2000) J. Invest. Dermatol. 115: 234
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G145)
Anti-Mouse CXCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: K125)
Anti-Rat CXCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: G121)
Anti-Mouse KC-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: K133)
Recombinant Mouse CXCL1 (aa 29 - 96) Truncated Form (Leinco Prod. No.: K134)
Anti-Mouse CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: K124)
Anti-Human CXCL1 (Leinco Prod. No.: C1393)
Anti-Mouse CXCL1 (Leinco Prod. No.: K135)
Anti-Human CXCL1 (Leinco Prod. No.: C1394)
Anti-Mouse CXCL1 (Leinco Prod. No.: C1420)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
