产品编号:V106
产品描述:Anti-Mouse VEGF-Biotin
反应种属:mouse
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5985.00
订购:
说明书:
Goat Anti-Mouse Vascular Endothelial Cell Growth Factor (VEGF)
Biotin
(VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen)
Polyclonal Antibody
Prod. No.: V106
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Vascular Endothelial Cell Growth Factor (VEGF) recognizes Mouse VEGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6
Known Species Cross Reactivity: Ms+
Immunogen
Sf 21-Derived VEGF164
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Mouse VEGF this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse VEGF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Folkman J et al. (2008) FASEB J. 22: 3728
2. Goodsell DS et al. (2002) The Oncologist 7: 569
3. Mugishima H. et al. (2006) J Atheroscler Thromb. 13: 130
4. Claesson-Welsh L et al. (2006) Exp Cell Res. 312: 549
5. Claesson-Welsh L et al. (1999) Trends Biochem Sci. 28: 488
6. Ellis LM et al. (2005) J Clin Oncol. 23: 1011
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse VEGF (Antigen Aff. Pur.) (Leinco Prod. No.: V104)
Anti-Human VEGF (Antigen Aff. Pur.) (Leinco Prod. No.: V100)
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Anti-Rat VEGF-Biotin (Antigen Aff.Pur.) (Leinco Prod. No.: V160)
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Anti-Rat VEGF (Leinco Prod. No.: V142)
Anti-Rat VEGF (Antigen Aff. Pur.) (Leinco Prod. No.: V149)
Recombinant Human VEGF 165 (Leinco Prod. No.: V101)
Human VEGF ELISA Development Kit (Leinco Prod. No.: V107)
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
