产品编号:S118
产品描述:Anti-Mouse SCF-Biotin
反应种属:mouse
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse Stem Cell Factor (SCF)
Biotin
(KITLG, Steel Factor (SF), DKFZp686F2250, KL-1, Kitl, Mast Cell Growth Factor (MGF))
Polyclonal Antibody
Prod. No.: S118
Host: Goat
Pkg. Size: 1mg, 50ug
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Stem Cell Factor (SCF) recognizes Mouse SCF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor1 is a paracrine regulator of germ cell development.2 SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.3 SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis4 and liver regeneration.5 SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.6
Known Species Cross Reactivity: Ms+
Immunogen
E. coli-Derived Recombinant Mouse SCF
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Mouse SCF this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse SCF is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Ian R et al. (2008) Culture of Animal Cells: A manual of basic technique
2. Toppari J et al. (1999) Endocrin 140: 1492
3. Adamson JW et al. (1991) PNAS 88: 7420
4. Hervé Lejeune et al. (2005) J of Endocrin. 186: 131
5. Ramadori G et al. (2009) Lab Invest.
6. Phan SH et al. (2009) AM J Pathol. 174: 390
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human SCF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: S113)
Anti-Rat SCF (Antigen Aff. Pur.) (Leinco Prod. No.: S512)
Anti-Mouse SCF (Antigen Aff. Pur.) (Leinco Prod. No.: S108)
Anti-Human SCF (Antigen Aff. Pur.) (Leinco Prod. No.: S107)
Anti-Rat SCF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: S513)
Recombinant Rat SCF (Leinco Prod. No.: S498)
Human Stem Cell Factor (SCF) ELISA Development Kit (Leinco Prod. No.: S122)
Anti-Human SCF (Leinco Prod. No.: S499)
Recombinant Human SCF (Leinco Prod. No.: S109)
Anti-Human SCF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: S588)
Anti-Mouse SCF (Leinco Prod. No.: S581)
Mouse SCF ELISA Development Kit (Leinco Prod. No.: S500)
Recombinant Mouse SCF (Leinco Prod. No.: S112)
Human SCF ELISA Development Kit (Leinco Prod. No.: S593)
Rat SCF ELISA Development Kit (Leinco Prod. No.: S594)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
