产品编号:M138
产品描述:Anti-Mouse MIP-1α-Biotin
反应种属:mouse
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse Macrophage Inflammatory Protein-1α (MIP-1α)
Biotin
(G0S19-1, LD78Alpha, MIP1A, SCYA3, CCL3)
Polyclonal Antibody
Prod. No.: M138
Host: Goat
Pkg. Size: 1mg, 50ug
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Macrophage Inflammatory Protein-1α (MIP-1α) recognizes Mouse MIP-1α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.1 MIP-1 alpha is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.2 The role of MIP-1 alpha seems to be related to production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.3
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse MIP-1α (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
References
1. IB Pragnell et al. (1994) J. Biol. Chem. 269: 4974
2. DN Cook et al. (1996) J. of Leukocyte Biol. 59: 61
3. PA Ward et al. (1995) J of Immunol. 154: 4793
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
