产品编号:M128
产品描述:Anti-Mouse MCP-5-Biotin
反应种属:mouse
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse Monocyte Chemotactic Protein-5 (MCP-5)
Biotin
(SCYA12, CCL12, Monocyte Chemoattractant Protein-5)
Polyclonal Antibody
Prod. No.: M128
Host: Goat
Pkg. Size: 1mg, 50ug
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Macrophage/Monocyte Chemotactic Protein-5 (MCP-5) recognizes Mouse MCP-5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Monocyte chemotactic protein 5 (MCP-5), also known as CCL 12, is a novel and potent monocyte active chemokine that is involved in allergic inflammation and the host response to pathogens.1 It is also a potent chemoattractant for peripheral blood monocytes and induces a calcium flux in peripheral blood mononuclear cells.2
Known Species Cross Reactivity: Ms+
Immunogen
E. coli-Derived Recombinant Mouse CCL12
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Mouse MCP-5 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for rmCCL12 is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Luster AD et al. (1997) J Exp Med. 185: 99
2. Jia GQ et al. (1996) J Exp Med. 184: 1939
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse MCP-5 (Leinco Prod. No.: C1413)
Anti-Mouse MCP-5 (Antigen Aff. Pur.) (Leinco Prod. No.: M149)
Recombinant Mouse MCP-5 (Leinco Prod. No.: M163)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
