产品编号:M149
产品描述:Anti-Mouse MCP-5
反应种属:mouse
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Monocyte Chemotactic Protein 5 (MCP-5)
Antigen Affinity Purified
(SCYA12, CCL12, Monocyte Chemoattractant Protein-5)
Polyclonal Antibody
Prod. No.: M149
Host: Goat
Pkg. Size: 1mg, 100ug
Description
Specificity:
Goat Anti-Mouse Monocyte Chemotactic Protein 5 (MCP-5) recognizes Mouse MCP-5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse MCP-5 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115). The detection sensitivity of this indirect ELISA for Mouse MCP-5 is approximately 0.1 ng/well.
Neutralization: This antibody is useful for neutralization of Mouse MCP-5 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse MCP-5 (at 0.2 ng/ml) is 0.8-4 µg/ml.
Western Blotting: To detect Mouse MCP-5 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse MCP-5 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse MCP-5 (Leinco Prod. No.: M163).
References
1. Luster AD et al. (1997) J Exp Med. 185: 99
2. Jia GQ et al. (1996) J Exp Med. 184: 1939
Related Products
Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse MCP-5 (Leinco Prod. No.: M163)
Anti-Mouse MCP-5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M128)
Anti-Mouse MCP-5 (Leinco Prod. No.: C1413)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
