产品编号:M126
产品描述:Anti-Mouse MCP-3-Biotin
反应种属:mouse
实验方法:WB
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse Monocyte Chemotactic Protein-3 (MCP-3)
Biotin
(CCL7, SCYA6, SCYA7, Small Inducible Cytokine A7, CCL7, NC28, Chemokine (C-C Motif) Ligand 7, FIC, MARC, MGC138463, MGC138465 )
Polyclonal Antibody
Prod. No.: M126
Host: Goat
Pkg. Size: 1 mg, 50µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Monocyte Chemotactic Protein-3 (MCP-3) recognizes Mouse MCP-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.1 MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes.
MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.2 MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.3,4,5
Known Species Cross Reactivity: Ms+
Immunogen
E. coli-Derived Recombinant Mouse MARC
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Mouse MCP-3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse MCP-3 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Van Damme J. et al. (1993) Biochem. Biophys. Res. Commun. 191: 535
2. Parmentier M. et al. (1999) Blood 94: 1899
3. Brosnan CF. et al. (1998) J Neuroimmunol. 86: 20
4. Reddigari SR. et al. (1995) Exp. Dermatol. 4: 260
5. Matikainen S. et al. (2001) Cytokine Growth Factor Rev. 12: 171
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human MCP-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M125)
Anti-Human MCP-3 (Antigen Aff. Pur.) (Leinco Prod. No.: M144)
Anti-Human MCP-3 (Leinco Prod. No.: M153)
Anti-Mouse CCL7 (Antigen Aff. Pur.) (Leinco Prod. No.: M175)
Recombinant Human MCP-3 (Leinco Prod. No.: M158)
Recombinant Mouse MCP-3 (Leinco Prod. No.: M134)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
