产品编号:L140
产品描述:Anti-Mouse CXCL5-Biotin
反应种属:mouse
实验方法:WB
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse CXCL5 Biotin
(AMCF-II, Epithelial-Derived Neutrophil-Activating Peptide 78 (ENA-78), GCP-2, Scyb5, Scyb6, Lipopolysaccharide-Induced CXC Chemokine (LIX))
Polyclonal Antibody
Prod. No.: L140
Host: Goat
Pkg. Size: 1mg, 50ug
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse CXCL5 recognizes Mouse CXCL5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
CXCL5 is an inflammatory chemokine that belongs to the CXC chemokine family1 that has a role in the development of cardiovascular diseases.2 CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils.1 It is expressed by epithelial cells within colorectal mucosa is a chemoattractant for neutrophils and has been implicated in Crohn's disease and ulcerative colitis.3 CXCL5 is important in growth and development of colorectal cancer, implicating a future role in both cancer therapy and diagnosis.4
Immunogen
E. coli-Derived Recombinant Mouse LIX
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Mouse LIX this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse LIX is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 400 ng/ml when used in conjunction with Anti-Mouse CXCL5 (Leinco Prod. No.: C1414) as the capture antibody at 4 µg/ml and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Mouse CXCL5. The sensitivity of this sandwich ELISA is 1 ng/ml.
References
1. Rajashekhar G et al. (2007) Physiol Genomics 31: 104
2. Mahsa M et al. (2008) Molec Diagnosis & Therapy 12: 391
3. Wågsäter Dick et al. (2007) International J Oncol. 31: 97
4. Fred Nagelkerke et al. (2008) Clinical Cancer Research 14: 2276
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse CXCL5 (Leinco Prod. No.: C1414)
Anti-Mouse CXCL5 (Antigen Aff. Pur.) (Leinco Prod. No.: L138)
Recombinant Mouse CXCL5 (Leinco Prod. No.: L123)
Anti-Human CXCL5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E122)
Anti-Human CXCL5 (Leinco Prod. No.: C1386)
Anti-Rat CXCL5 (Antigen Aff. Pur.) (Leinco Prod. No.: L221)
Anti-Rat CXCL5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L231)
Anti-Human CXCL5 (Antigen Aff. Pur.) (Leinco Prod. No.: E106)
Anti-Rat CXCL5 (Leinco Prod. No.: L217)
Anti-Human CXCL5 (Leinco Prod. No.: C1607)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
