产品编号:I-276
产品描述:Anti-Mouse CXCL10-Biotin
反应种属:mouse
实验方法:WB, ELISA., ICC
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse Chemokine (C-X-C Motif) Ligand 10 (CXCL10)
Biotin
(C7, IFI10, INP10, IP-10, SCYB10, Crg-2, GIP-10, Mob-1)
Polyclonal Antibody
Prod. No.: I-276
Host: Goat
Pkg. Size: 1mg, 50ug
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Chemokine (C-X-C Motif) Ligand 10 (CXCL10) recognizes Mouse CXCL10. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family. CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts.1 CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.2,3 CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury.4
Known Species Cross Reactivity: Ms+
Immunogen
E. coli-Derived Recombinant Mouse CXCL10
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, IHC (NBF/Par.), WB,
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 - 15 µg/ml. The recommended secondary antibody for IHC is UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Mouse IP-10 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse IP-10 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 600 ng/ml when used in conjunction with Anti-Mouse CXCL10 (Leinco Prod. No.: C1423) as the capture antibody at 100 µl/well and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Mouse CXCL10. The sensitivity of this sandwich ELISA is 4 ng/ml.
References
1. Luster et al.(1985) Nature 315: 672
2. Dufour et al.(2002) J. Immunol. 168
3. Angiolillo et al.(1995) J. Exp. Med. 182: 155
4. Kaneko S. et al.(2008) Nephron Exp. Nephrol. 109: c29
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse CXCL10 (Leinco Prod. No.: C1423)
Anti-Human CXCL10-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-275)
Anti-Human CXCL10 (Antigen Aff. Pur.) (Leinco Prod. No.: I-160)
Anti-Mouse CXCL10 (Antigen Aff. Pur.) (Leinco Prod. No.: I-236)
Anti-Cotton Rat CXCL10 (Antigen Aff. Pur.) (Leinco Prod. No.: I-601)
Anti-Human CXCL10 (Leinco Prod. No.: C1389)
Human CXCL10 ELISA Development Kit (Leinco Prod. No.: I-423)
Recombinant Human CXCL10 (Leinco Prod. No.: I-202)
Recombinant Mouse IP-10 (Leinco Prod. No.: I-229)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
