产品编号:I-365

产品描述:Anti-Mouse IL-18BPc-Biotin

反应种属:mouse

实验方法:WB

标记:

规格:50ug

供应商:leinco

价格(RMB):5985.00

订购:

说明书:

Goat Anti-Mouse Interleukin-18 Binding Protein (IL-18BPc)
Biotin
Polyclonal Antibody
Prod. No.: I-365
Host:      Goat
Pkg. Size: 1 mg, 50 µg
Storage:   -20°C to -70°C

Description
Specificity:
Goat Anti-Mouse Interleukin-18 Binding Protein (IL-18BPc) recognizes Mouse IL-18BPc. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
IL-18BP is a circulating glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 with high affinity and neutralizing its biological activity. IL-18BP has no amino acid sequence homology to IL-18 or IL-1 receptors. The gene for human IL-18BP encodes at least four isoforms by alternative splicing. The IL-18BP isoforms A and C each contain one immunoglobulin (Ig)-like C2-type domain while isoforms B and D lack the complete Ig domain. The complete Ig domain has been shown to be essential for IL-18BPs binding and neutralizing properties. Several poxviruses have been shown to encode proteins with sequence similarity to human and mouse IL-18BPs that also bind and inhibit IL-18 bioactivities. This could lead to viral modulation of host immune responses.

Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse IL-18BP (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB

Related Products
Anti-Mouse IL-18BP (Antigen Aff. Pur.) (Leinco Prod. No.: I-364)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.